Journal: Frontiers in Endocrinology

Article Title: Role of Moesin Phosphorylation in Retinal Pericyte Migration and Detachment Induced by Advanced Glycation Endproducts

doi: 10.3389/fendo.2020.603450

Figure Lengend Snippet: Advanced glycation-bovine serum albumin (AGE-BSA)-induced moesin phosphorylation and RMP migration involve Rho-associated protein kinase (ROCK). Y27632, specific inhibitor of ROCK (10 μmol/L), was pre-incubated 1 h before AGE-BSA (100 μg/ml, 24 h) application. (A) Moesin phosphorylation and total moesin expression in RMPs were detected using immunoblotting. The cropped images represent blotting experiments that were performed under the same experimental conditions. (B, C) Cell migration was detected using scratch wound healing and transwell assays. (D) Cell viability. Results shown are representative experiment and quantitative results. N = 3 independent experiments. * p < 0.05 vs . control, # p < 0.05 vs . AGE-BSA.

Article Snippet: ROCK inhibitor Y27632 was from TargetMol (USA).

Techniques: Migration, Incubation, Expressing, Western Blot

Journal: Frontiers in Endocrinology

Article Title: Role of Moesin Phosphorylation in Retinal Pericyte Migration and Detachment Induced by Advanced Glycation Endproducts

doi: 10.3389/fendo.2020.603450

Figure Lengend Snippet: Advanced glycation-bovine serum albumin (AGE-BSA) up-regulates the expression of CD44 and its binding to phospho-moesin. (A) Cultured retinal microvascular pericytes (RMPs) were treated with AGE-BSA (100 μg/ml, 24 h) and subjected to immunostaining with antibodies to CD44 (green). (B, C) Cultured RMPs were treated with AGE-BSA or Y27632 + AGE-BSA and the expression of CD44 was detected using immunoblotting. CLSM colocalization studies were performed for phospho-moesin (red) and CD44 (green). (D) RMPs treated with or without AGE-BSA were lysed and subjected to immunoprecipitation (IP) with antibodies against CD44 or with control IgG. The resulting precipitates as well as the cell lysates were subjected to immunoblot analysis with antibodies against phospho-moesin or CD44. Results shown are representative experiment and quantitative results. N = 3 independent experiments. * p < 0.05 vs . control. Scale bar, 30 μm.

Article Snippet: ROCK inhibitor Y27632 was from TargetMol (USA).

Techniques: Expressing, Binding Assay, Cell Culture, Immunostaining, Western Blot, Immunoprecipitation

Journal: bioRxiv

Article Title: Targeting ROCK Signaling Mitigates Influenza Virus-Induced Fibrogenesis in Human Airway Organoids

doi: 10.1101/2025.04.21.649854

Figure Lengend Snippet: (A) HAO tissue was infected (MOI 0.001) with HPh-TX H5N1 and treated with a non-toxic dose (5 μM) of ROCK1 (GSK269962) or ROCK2 (KD025, Belumosudil) inhibitors from 5-DPI to 10-DPI. HAO tissue was fixed and stained with α-SMA (green) at 10-DPI. (B-D) Gene expression of extracellular matrix proteins fibronectin (FN), collagen 1A (COL1A), and collagen 3A (COL3A). ( E , F ) Gene expression of tissue remodeling MMP2 and MMP9. Data were analyzed using One-way ANOVA followed by a post-test comparison. The significant differences are indicated (* = p < 0.05, ** = p < 0.01, *** = p < 0.001; non-significant = ns).

Article Snippet: GSK269962A (GSK 269962), the ROCK1 selective inhibitor (CAS No: 850664-21-0), and Belumosudil (KD025; SLx-2119), the ROCK2 selective inhibitor (CAS No: 911417-87-3), were purchased from MedChemExpress (NJ, USA).

Techniques: Infection, Staining, Gene Expression, Comparison